Coding

Part:BBa_K4137001:Design

Designed by: Clifford S Chang   Group: iGEM22_GEMS_Taiwan   (2022-08-20)


CcdB-Myc Toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

A Myc-tag was added to the C-terminal of the protein for purification purposes; Myc was selected to distinguish CcdB from CcdA. This sequence has been codon optimized for E coli.

Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1

References

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1