Coding
Part:BBa_K4137001:Design
Designed by: Clifford S Chang Group: iGEM22_GEMS_Taiwan (2022-08-20)
CcdB-Myc Toxin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 216
Design Notes
A Myc-tag was added to the C-terminal of the protein for purification purposes; Myc was selected to distinguish CcdB from CcdA. This sequence has been codon optimized for E coli.
Source
https://www.ncbi.nlm.nih.gov/nuccore/U51588.1